Capillary Electrophoresis of Proteins and Peptides (Methods in Molecular Biology (Clifton, N.J.), V. 276.) (Methods in Molecular Biology) Review

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(More customer reviews)Obviously most of us will be introduced to this subject through a vendor and an expensive "turn-key" system, but the editors and contributors to this volume did an excellent job of summarizing a variety of approaches to the theory and applications of CE.This field is obviously still ahead of it's time, mostly academic, and a great topic for graduate research.Certainly many applications have been replaced with more sophisticated techniques like mass spectrometry, but for those with even minimal time, it's great to consider separation inside of a very small tube by several forces simultaneously, very quickly and with extremely high resolution.Also, the physical and chemical environment lend themselves to experimental designs involving many kinds of interactions on a chemical level (such as complexation with ligands, even including colorimetric applications which might not be as simple with ordinary liquid chromatography) over an extremely fast time scale.This may be a nice book that is only appreciated after another decade passes and industry has actually hired enough innovative scientists that are daring enough to try some experiments on their own time and force some necessary cultural changes.

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Expert academic andpharmaceutical researchers describe their best capillary electrophoresis techniques for protein and peptide analysis. The authors present cutting-edge techniques for both capillary coatings and analytic detection via laser-induced fluorescence, for the development and commercialization of biopharmaceuticals, and for affinity capillary electrophoresis in the evaluation of protein binding, including the use of protein charge ladders. Areas of special interest covered include combining CE and capillary isoelectric focusing with electrospray mass spectrometry detection to perform proteomic studies and applications in the field of protein-ligand binding.

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