Gene Transfer: Delivery and Expression of DNA And RNA, A Laboratory Manual Review

Average Reviews:

(More customer reviews)This book describes a series of protocols that offer ways to deliver genes into mammalian cells. About half the book describes viral techniques where a virus is used to do the transport and delivery. The other half describes non-viral methods.The book has some 75 different techniques, each described by a researcher who has been using/developing that method.

Note that many of these techniques are brand new and still in the stage of being developed. Some of them are likely to prove useful, others will be of limited use, perhaps in a niche application or will simply lead to better techniques later on.

Dr. Rossi (Bechmann Research Institute) and Dr. Friedmann (UCSD) serve as editors of the book. It is called a Laboratory Manual as the protocols are presented in a stepwise, 'cookbook' style.

The book was published in early 2007 and should be considered an accurate reflection of the state of the art as of late in 2006.

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Product Description:
Understanding gene function and regulation requires rigorous testign in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of avaiable techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, trans genic approaches, strategies for the regulation of trransgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions wioth considerations for selection of appropriate system and strategies for delivery..Related Titles from the Publisher Binding and Kinetics for Molecular Biologists; DNA Replication and Human Disease; DNA Science; Epigenetics; Genes and Signals; Genetic Variation; Genomes; Molecular Biology of the Gene, 6th Edition; Recombinant DNA: Genes and Genomes - A Short Course